A Real-Time PCR Antibiogram for Drug-Resistant Sepsis
نویسندگان
چکیده
منابع مشابه
A Real-Time PCR Antibiogram for Drug-Resistant Sepsis
Current molecular diagnostic techniques for susceptibility testing of septicemia rely on genotyping for the presence of known resistance cassettes. This technique is intrinsically vulnerable due to the inability to detect newly emergent resistance genes. Traditional phenotypic susceptibility testing has always been a superior method to assay for resistance; however, relying on the multi-day gro...
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Mutations in particular nucleotides of genes coding for drug targets or drug-converting enzymes lead to drug resistance in Mycobacterium tuberculosis. For rapid detection of drug-resistant M. tuberculosis in clinical specimens, a simple and applicable method is needed. Eight TaqMan minor groove binder (MGB) probes, which discriminate one-base mismatches, were designed (dual-probe assay with fou...
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1 Early diagnosis of drug resistant Mycobacterium tuberculosis is urgently needed to 2 optimize treatment regimens and to prevent the transmission of resistant strains. Real3 time PCR assays have been developed to detect drug resistance rapidly, but none of them 4 have been widely applied due to their complexity, high cost, or requirement for advanced 5 instruments. In this study, we developed ...
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Early diagnosis of drug-resistant Mycobacterium tuberculosis is urgently needed to optimize treatment regimens and to prevent the transmission of resistant strains. Real-time PCR assays have been developed to detect drug resistance rapidly, but none of them have been widely applied due to their complexity, high cost, or requirement for advanced instruments. In this study, we developed a real-ti...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2011
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0028528